Structure of the Roc–COR domain tandem of C. tepidum, a prokaryotic homologue of the human LRRK2 Parkinson kinase
Identifieur interne : 000C64 ( Main/Exploration ); précédent : 000C63; suivant : 000C65Structure of the Roc–COR domain tandem of C. tepidum, a prokaryotic homologue of the human LRRK2 Parkinson kinase
Auteurs : Katja Gotthardt [Allemagne] ; Michael Weyand [Allemagne] ; Arjan Kortholt [Allemagne, Pays-Bas] ; Peter J M. Van Haastert [Pays-Bas] ; Alfred Wittinghofer [Allemagne]Source :
- The EMBO Journal [ 0261-4189 ] ; 2008-08-20.
Abstract
Ras of complex proteins (Roc) belongs to the superfamily of Ras‐related small G‐proteins that always occurs in tandem with the C‐terminal of Roc (COR) domain. This Roc–COR tandem is found in the bacterial and eukaryotic world. Its most prominent member is the leucine‐rich repeat kinase LRRK2, which is mutated and activated in Parkinson patients. Here, we investigated biochemically and structurally the Roco protein from Chlorobium tepidum. We show that Roc is highly homologous to Ras, whereas the COR domain is a dimerisation device. The juxtaposition of the G‐domains and mutational analysis suggest that the Roc GTPase reaction is stimulated and/or regulated by dimerisation in a nucleotide‐dependent manner. The region most conserved between bacteria and man is the interface between Roc and COR, where single‐point Parkinson mutations of the Roc and COR domains are in close proximity. The analogous mutations in C. tepidum Roc–COR decrease the GTPase reaction rate, most likely due to a modification of the interaction between the Roc and COR domains.
Url:
DOI: 10.1038/emboj.2008.150
Affiliations:
- Allemagne, Pays-Bas
- District d'Arnsberg, Groningue (province), Rhénanie-du-Nord-Westphalie
- Dortmund, Groningue (ville)
- Université de Groningue
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structure of the Roc–COR domain tandem of C. tepidum, a prokaryotic homologue of the human LRRK2 Parkinson kinase</title><author><name sortKey="Gotthardt, Katja" sort="Gotthardt, Katja" uniqKey="Gotthardt K" first="Katja" last="Gotthardt">Katja Gotthardt</name></author><author><name sortKey="Weyand, Michael" sort="Weyand, Michael" uniqKey="Weyand M" first="Michael" last="Weyand">Michael Weyand</name></author><author><name sortKey="Kortholt, Arjan" sort="Kortholt, Arjan" uniqKey="Kortholt A" first="Arjan" last="Kortholt">Arjan Kortholt</name></author><author><name sortKey="Van Haastert, Peter J M" sort="Van Haastert, Peter J M" uniqKey="Van Haastert P" first="Peter J M" last="Van Haastert">Peter J M. Van Haastert</name></author><author><name sortKey="Wittinghofer, Alfred" sort="Wittinghofer, Alfred" uniqKey="Wittinghofer A" first="Alfred" last="Wittinghofer">Alfred Wittinghofer</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:31CEAE960800F7052FFD4321C24D8EC3BF392087</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1038/emboj.2008.150</idno><idno type="url">https://api.istex.fr/document/31CEAE960800F7052FFD4321C24D8EC3BF392087/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">001A96</idno><idno type="wicri:Area/Main/Curation">001835</idno><idno type="wicri:Area/Main/Exploration">000C64</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Structure of the Roc–COR domain tandem of C. tepidum, a prokaryotic homologue of the human LRRK2 Parkinson kinase</title><author><name sortKey="Gotthardt, Katja" sort="Gotthardt, Katja" uniqKey="Gotthardt K" first="Katja" last="Gotthardt">Katja Gotthardt</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Structural Biology, Max‐Planck‐Institut for Molecular Physiology, Dortmund</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District d'Arnsberg</region><settlement type="city">Dortmund</settlement></placeName></affiliation></author><author><name sortKey="Weyand, Michael" sort="Weyand, Michael" uniqKey="Weyand M" first="Michael" last="Weyand">Michael Weyand</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Structural Biology, Max‐Planck‐Institut for Molecular Physiology, Dortmund</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District d'Arnsberg</region><settlement type="city">Dortmund</settlement></placeName></affiliation></author><author><name sortKey="Kortholt, Arjan" sort="Kortholt, Arjan" uniqKey="Kortholt A" first="Arjan" last="Kortholt">Arjan Kortholt</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Structural Biology, Max‐Planck‐Institut for Molecular Physiology, Dortmund</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District d'Arnsberg</region><settlement type="city">Dortmund</settlement></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Molecular Cell Biology, University of Groningen, NN Haren</wicri:regionArea><orgName type="university">Université de Groningue</orgName><placeName><settlement type="city">Groningue (ville)</settlement><region>Groningue (province)</region></placeName></affiliation></author><author><name sortKey="Van Haastert, Peter J M" sort="Van Haastert, Peter J M" uniqKey="Van Haastert P" first="Peter J M" last="Van Haastert">Peter J M. Van Haastert</name><affiliation wicri:level="4"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Molecular Cell Biology, University of Groningen, NN Haren</wicri:regionArea><orgName type="university">Université de Groningue</orgName><placeName><settlement type="city">Groningue (ville)</settlement><region>Groningue (province)</region></placeName></affiliation></author><author><name sortKey="Wittinghofer, Alfred" sort="Wittinghofer, Alfred" uniqKey="Wittinghofer A" first="Alfred" last="Wittinghofer">Alfred Wittinghofer</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Structural Biology, Max‐Planck‐Institut for Molecular Physiology, Dortmund</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District d'Arnsberg</region><settlement type="city">Dortmund</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">The EMBO Journal</title><idno type="ISSN">0261-4189</idno><idno type="eISSN">1460-2075</idno><imprint><publisher>John Wiley & Sons, Ltd</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2008-08-20">2008-08-20</date><biblScope unit="volume">27</biblScope><biblScope unit="issue">16</biblScope><biblScope unit="page" from="2239">2239</biblScope><biblScope unit="page" to="2249">2249</biblScope></imprint><idno type="ISSN">0261-4189</idno></series><idno type="istex">31CEAE960800F7052FFD4321C24D8EC3BF392087</idno><idno type="DOI">10.1038/emboj.2008.150</idno><idno type="ArticleID">EMBJ2008150</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0261-4189</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Ras of complex proteins (Roc) belongs to the superfamily of Ras‐related small G‐proteins that always occurs in tandem with the C‐terminal of Roc (COR) domain. This Roc–COR tandem is found in the bacterial and eukaryotic world. Its most prominent member is the leucine‐rich repeat kinase LRRK2, which is mutated and activated in Parkinson patients. Here, we investigated biochemically and structurally the Roco protein from Chlorobium tepidum. We show that Roc is highly homologous to Ras, whereas the COR domain is a dimerisation device. The juxtaposition of the G‐domains and mutational analysis suggest that the Roc GTPase reaction is stimulated and/or regulated by dimerisation in a nucleotide‐dependent manner. The region most conserved between bacteria and man is the interface between Roc and COR, where single‐point Parkinson mutations of the Roc and COR domains are in close proximity. The analogous mutations in C. tepidum Roc–COR decrease the GTPase reaction rate, most likely due to a modification of the interaction between the Roc and COR domains.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Pays-Bas</li></country><region><li>District d'Arnsberg</li><li>Groningue (province)</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Dortmund</li><li>Groningue (ville)</li></settlement><orgName><li>Université de Groningue</li></orgName></list><tree><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Gotthardt, Katja" sort="Gotthardt, Katja" uniqKey="Gotthardt K" first="Katja" last="Gotthardt">Katja Gotthardt</name></region><name sortKey="Kortholt, Arjan" sort="Kortholt, Arjan" uniqKey="Kortholt A" first="Arjan" last="Kortholt">Arjan Kortholt</name><name sortKey="Weyand, Michael" sort="Weyand, Michael" uniqKey="Weyand M" first="Michael" last="Weyand">Michael Weyand</name><name sortKey="Wittinghofer, Alfred" sort="Wittinghofer, Alfred" uniqKey="Wittinghofer A" first="Alfred" last="Wittinghofer">Alfred Wittinghofer</name></country><country name="Pays-Bas"><region name="Groningue (province)"><name sortKey="Kortholt, Arjan" sort="Kortholt, Arjan" uniqKey="Kortholt A" first="Arjan" last="Kortholt">Arjan Kortholt</name></region><name sortKey="Van Haastert, Peter J M" sort="Van Haastert, Peter J M" uniqKey="Van Haastert P" first="Peter J M" last="Van Haastert">Peter J M. Van Haastert</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C64 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000C64 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:31CEAE960800F7052FFD4321C24D8EC3BF392087
|texte= Structure of the Roc–COR domain tandem of C. tepidum, a prokaryotic homologue of the human LRRK2 Parkinson kinase
}}
| This area was generated with Dilib version V0.6.23. |  |